SubtiBank SubtiBank
Version comparison:

2018-02-16 12:23:472025-04-04 20:04:55

description

penicillin-binding protein 4

class A penicillin-binding protein 4

locus

BSU31490

BSU_31490

geneLength

1872

1875

ec

outlinks

bsu

BSU31490

BSU_31490

The protein

[SW|Localization]

cell membrane (according to Swiss-Prot)

cell membrane (according to UniProt)

during vegetative growth: septal, distinct spots at periphery [pubmed|14731276]

References

14731276, 8606187, 7961491, 20487272, 28045164, 19458048

14731276, 8606187, 7961491, 20487272, 28045164, 19458048, 29456081, 26499795

Gene

Phenotypes of a mutant

a strain lacking all four class A [SW|penicillin-binding proteins] ([[gene|ponA]] [[gene|pbpD]] [[gene|pbpF]] [[gene|pbpG]]) is severely inhibited for L-form switching in the presence of D-cycloserine [pubmed|29456081]

The protein

Catalyzed reaction/ biological activity

highly active transpeptidase, responsible for about 50% of D-amino acid incorporation [pubmed|26499795]

[GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)](n)-diphospho-di-trans,octa-cis-undecaprenol + β-D-GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-di-trans,octa-cis-undecaprenol --> [GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-diphospho-di-trans-octa-cis-undecaprenol + di-trans,octa-cis-undecaprenyl diphosphate + H+ (according to UniProt)

Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine (according to UniProt)

The protein

Protein family

N-terminal part: [SW|glycosyltransferase 51 family] (according to UniProt)

C-terminal part: [SW|transpeptidase family] (according to UniProt)

The protein

Paralogous protein(s)

[[protein|PbpF]], [[protein|PbpG]], [[protein|PonA]]

Biological materials

GFP fusion

2082 [[gene|trpC]]2 [[gene|pbpD]]::pSG1490 (cat Pxyl-gfpa-[[gene|pbpD]]1-510) [pubmed|14731276], available in [SW|Dirk Jan Scheffers]' lab and in the [http://bgsc.org BGSC]